Analysis Note
λmax. ~428 nm/~392 nm; E(1%,1 cm) ~275-425/~250-400 (H2O)
Application
Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.
Biochem/physiol Actions
Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.
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